Keywords

• DNA ligase (ATP)
• Synthetase,polydeoxyribonucleotide (adenosine triphosphate)
• DNA LIGASE

Quick Details

• ProName: T4 DNA Ligase CAS 9015-85-4
• CasNo: 9015-85-4
• Appearance: powder
• Application: Reagent for biochemical purposes
• DeliveryTime: 7-10 working days after deposit
• PackAge: Inside plastic outside
• Port: Tianjin or Qingdao
• ProductionCapacity: 200 Metric Ton/Week
• Purity: 98%
• Storage: Store in a cool and dry place
• Transportation: We ship goods via DHL, Fedex, UPS, TNT...
• LimitNum: 1 Kilogram

Superiority

With our good experience, we offer detailed technical support and advice to assist customers. We communicate closely with customers to establish their quality requirements.

Consistent Quality
Our plant has strict quality control in each manufacturing process. Guaranteed.
Meanwhile all the materials are tested before each shipment in our laboratory to double confirm the quality.

Most competitive pricing
Our company is committed to the production and development of titanium dioxide, we always offer the most competitive pricing to customers to help lower their cost in application.

Shortest delivery time
90% of orders are shipped within 7-10 days after receipt of the prepayment or workable L/C.

Respond in 24 hours to inquiry, feedback or other requirements
Our experienced staff is dedicated to answer all of your inquires, feedback or other requirements in 24 hours.

We welcome you to contact us for more information and look forward to working with yo

Details

Our company specializes in processing and selling chemical raw materials, chemical products, pharmaceutical intermediates, veterinary medicine intermediates, dye intermediates, pigments, cosmetics raw materials and chemical reagents,  
Our company has a number of sales team, service and information feedback vertical integration of a sound marketing network, products are sold throughout the country and exported to South Asia, Europe, America, Africa and other more than 20 countries and regions.  
Companies adhering to the "pursuit of quality, innovation and development" business philosophy, to serve as a foundation, for the survival by the quality, seek development by science and technology, adhere to quilty comes first,fair price,good sense of customer service and responsibility. in the future hebei Nengqian import and export trade Co.,Ltd will always hold "integrity, innovation" business philosophy, to achieve honesty management, on the way of specialization, internationalization,  Keep moving!  
Our company's strengths：
1.Our products are superior in quality and competitive in price
2. Standard: BP/USP/EP/ enterprise standard
All purities ≥99%
3. Fast and safe delivery and careful delivery, a variety of transportation modes for your choice of service.A clearance rate of 99% or higher

Product Description:

CAS : 9015-85-4
Molecular weight: 55.3kDa, monomer
Grade: BR
Source: Escherichia coli containing T4 phage clone gene 30
Concentration: 5Weiss u/ul (1000CEU/ul)
Activity definition: refers to the amount of enzyme required to convert 1nmol [32PPi] to Norit absorbable form within 30 minutes at 37°C in the ATP-PPi exchange reaction (weiss units, 1 weiss unit is equivalent to about 200 sticky ends Linking unit. 1 sticky end linking unit: 20ul reaction system (50mM Tris-HCL (PH7.5), 10mM DTT, 10mM MgCL2, 1mM ATP, 25ug/ml BSA, 12uM (300ug/ml) 5-DNA end) , The amount of enzyme required to ligate 50% of the ligated Lambda DNA product digested with HindIII within 30 minutes at 16°C)
Enzyme activity analysis mixture: 66mM Tris-HCL (PH7.6), 10mM DTT, 6.6mM MgCL2, 0.066mM ATP, 3.3uM [32PPi]
Storage solution components: 20mM Tris-HCL (PH7.5), 50mM KC1, 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol
10×T4 DNA Ligase buffer (#B69): 400mM Tris-HCL, 100mM MgCL2, 100mM DTT, 5mM ATP (PH7.5, 25ºC)
Inhibitor: When the concentration of NaC1 or KC1 in the reaction system exceeds 200 mM, it can strongly inhibit T4 DNA Ligase activity
Inactivation: heating at 65ºC for 10 minutes or 70ºC for 5 minutes
Note: The binding of T4 DNA Ligase to DNA will change the band agarose gel electrophoretic mobility. To avoid this phenomenon, samples or molecular weight standards need to be processed before electrophoresis. The sample processing is as follows: the sample is mixed with Fermentas 6×DNA Loading Dye&SDS solution (#R1151), heated at 75°C for 5 minutes or 65°C for 10 minutes and stored in an ice bath; during transformation, the volume of the ligation product must not exceed the volume of competent cells 10%; before electrotransformation, remove the T4 DNA Ligase in the ligation mixture by extraction method, and then purify the extraction product by ethanol precipitation
Quality control: Tests show no endonuclease, ribonuclease, and phosphatase contamination. Functional test: the ability to connect DNA with sticky or blunt ends
Traits: liquid. This enzyme catalyzes the formation of a phosphodiester bond between the adjacent 5'phosphate group and 3'-hydroxyl end in double-stranded DNA or RNA, and can also repair single-stranded cuts in double-stranded DNA, RNA, or DNA/RNA complexes. The sticky and flat ends of the DNA are connected, but the enzyme has no enzyme activity on single-stranded nucleic acids. This enzyme requires the cofactor ATP
Uses: biochemical research. Linkage of sticky-end or blunt-end double-stranded DNA; double-stranded oligonucleotide linker to double-stranded DNA; repair of gaps in double-stranded DNA, RNA, or DNA-RNA complexes; ligase-mediated RNA detection; sites Specific mutation; amplified fragment length polymorphism
Storage: -20°C

Packing:  
  1. Inner double plastic bags----25kg/Fiber drum (35*35*45cm, GW: 28kg, NW: 25kg); 

  2. Inner double plastic bags----5kg/Aluminum foil bag (GW: 6.5kg, NW: 5kg). 

  3. Inner double plastic bags----1kg/Aluminum foil bag (GW: 1.5kg, NW: 1kg). 

P.S.: we accept packaging customization. 
  
Delivery:   

  1. Stock products are normally shipping within 3 working days.  

  2. For rush delivery, please contact our sales team for particular arrangement. 

Shipping Details: 
  1. We ship goods via DHL, Fedex, UPS, TNT, China post, NL Post and other couriers, weight from 10g to 1000kg or even bulker. 

  2. Shipping details & shipping documents will be provided and sent by email. 

  3. We keep tracking the shippment until clients well received. 

  4. After years export shipping experience, with professional and experienced cooperated forwarders, we ensure that goods can be delivered in multiple ways safetly and efficiently. 

FAQ:
Q1:Will you supply samples for testing
A: Free samples for the quality evaluation for most products, but customers only need to undertake the shipping fee for delivery the samples to your door.

Q2:What's your MOQ
A: For the high value product, our MOQ starts from 10g,100g and 1kg.

Q3:Which kind of payment terms do you accept
A: Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T, and BTC). Bulk order by T/T or L/C.

Q4:How about your delivery time
A: Generally, it will take 3 to 5 days after receiving your advance payment.

Q5:How do you treat quality complaint
A:First of all, our quality control will reduce the quality problem to near zero. If there is a real quality problem caused by us, we will send you free goods for replacement or refund your loss.